Botulinum toxin is administered by diluting the powder in saline (sodium chloride) and injecting it directly into neuromuscular tissue. It takes 24-72 hours for botulinum toxin to take effect, which reflects the time needed for the toxin to disrupt the synaptosomal process. In very rare circumstances, it may take as long as 5 days for the full effect of botulinum toxin to be observed.Read more about BOTOX at http://botoxonline.co.